Intestinal nipi-3 expression is necessary and sufficient for resistance against ToxA and P. aeruginosa.
a Lifespans of gut or hypodermal RNAi strains fed E. coli expressing ToxA (left) or P. aeruginosa PA14 (right) following either nipi-3 or L4440 vector control RNAi. P < 0.001 for gut RNAi assays; P > 0.02 for hyp RNAi assays (log-rank test). b qRT-PCR comparison of L4 animals following nipi-3 RNAi started at the L1 stage. Results shown are an average of four biological replicates, each normalized to the corresponding wild type L4440 control values. Error bars represent SEM. *P < 0.05 compared to corresponding wild type animals (Student’s unpaired t-test). c Lifespans of wild type N2, two independent lines of nipi-3(fr4) expressing intestinal vha-6p::nipi-3, and the non-transgenic offspring of nipi-3(fr4);agEx122 fed E. coli expressing ToxA. Number of animals scored for each condition was > 50 (373 total; a ToxA), ≥ 145 (656 total; a
P. aeruginosa), and ≥ 130 (558 total; c). These are representative experiments of four (a ToxA), three (a
P. aeruginosa), or two (c) independent experiments. Primary data for panel b are provided in Additional file 15