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Fig. 4 | BMC Biology

Fig. 4

From: Tribbles ortholog NIPI-3 and bZIP transcription factor CEBP-1 regulate a Caenorhabditis elegans intestinal immune surveillance pathway

Fig. 4

NIPI-3 is not immediately up or downstream of PMK-1 p38 MAPK or KGB-1 JNK-like pathways. a Western blot analysis of phosphorylated PMK-1 in L4 animals of the indicated genotype raised on control OP50 E. coli. b Transcript fold changes determined by microarray analysis of nipi-3(fr4) or pmk-1(km25) animals as compared to wild type N2 raised on OP50 E. coli. Only genes significantly altered in nipi-3(fr4) are shown. c Lifespans of wild type N2 and pmk-1(km25) (left) or kgb-1(km21) (right) fed E. coli expressing ToxA following either nipi-3 or L4440 vector control RNAi. P < 0.001 comparing pmk-1 and pmk-1, nipi-3 and comparing kgb-1 and kgb-1; nipi-3 (log-rank test). Number of animals scored for each condition was ≥ 55 (278 total; c right) and > 90 (424 total; c left). These are representative experiments of two (c left) or three (c right) independent experiments. Primary data for panel b are provided in Additional file 3: Table S2

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