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Fig. 2 | BMC Biology

Fig. 2

From: Arabidopsis PRC1 core component AtRING1 regulates stem cell-determining carpel development mainly through repression of class I KNOX genes

Fig. 2

AtRING1b displays maternally imprinted expression in endosperm. ad AtRING1b expression was analyzed in the seeds after reciprocal crosses of AtRING1b::AtRING1b-GUS (Columbia, Col background) with Col, fis2, or met1-3 at 3 DAP. a AtRING1b::AtRING1b-GUS (♀) × Col (♂). Similar patterns were observed for AtRING1b::AtRING1b-GUS (♀) × fis2 (♂) and AtRING1b::AtRING1b-GUS (♀) × met1-3. (b) Col (♀) × AtRING1b::AtRING1b-GUS (♂). c fis2 (♀) × AtRING1b::AtRING1b-GUS (♂). d met1-3 (♀) × AtRING1b::AtRING1b-GUS (♂). eh Expression pattern of AtBMI1c investigated by analysis of AtBMI1c::AtBMI1c-GUS transgenic lines. e Staining in RAM (inset) and junction between root and shoot of 1-week-old seedling. f Developing anther. g Fertilized ovule at 1 DAP. h Developing seed at globular stage. il Expression pattern of AtBMI1c in atring1a;atring1b mutant in AtBMI1c::AtBMI1c-GUS lines. i Embryo-like structure produced in 2-week-old atring1a;atring1b seedling. j Inflorescence. k Developing gynoecium (about stage 9). l Developing gynoecium (about stage 12) and young ovule (inset). m Increased levels of BMI1 transcripts in the atring1a;atring1b mutant detected by qRT-PCR (Student’s t test, *p < 0.05, **p < 0.01). Error bars represent SD for three biological replicates. nq AtBMI1c expression analyzed in the AtBMI1c::AtBMI1c-GUS construct in seeds after reciprocal crosses, as described in ad for AtRING1b::AtRING1b-GUS. Arrowheads indicate chalazal endosperm. Bars = 100 μm, except 500 μm in e and i, and 50 μm in f

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