Fig. 5

Expression pattern of WUS-AG and KNOX-I pathway-related genes in atring1a;atring1b mutant. a Expression profiles of select key genes of both stem-cell determining pathways and three AG-related genes in floral buds of atring1a;atring1b mutant detected using qRT-PCR (Student’s t test, **p < 0.01). Error bars represent SD from three biological replicates. b, c LFY expression pattern detected using LFY::GUS reporter. b No staining in WT developing carpel. c Strong staining in developing carpel (c1) and in extra floral organs between petal and carpel (c2) in atring1a;atring1b mutant. d, e WUS expression pattern detected using WUS::GUS reporter. d Staining in the WT floral meristem and in nucellus of developing ovule (inset). e Staining in floral meristem (e1) and in nucellus of developing primary (e2) and secondary ovules (e3) in atring1a;atring1b mutant. f, g AG::GUS reporter showing that WT (f) has stronger staining in developing carpel and anther compared to atring1a;atring1b mutant (g). Arrow indicates signal in ectopic stigma papillae in sepal. h, i CLV3 expression pattern detected using CLV3::GUS reporter. h Staining in the WT floral meristem, but not in ovule primordium (inset). i Staining in FM and developing carpel (i1), in the ectopic papillae of carpelloid sepal (i2), and in nucellus of developing ovule (i3 and i4) in atring1a;atring1b mutant. j, k STM expression detected using STM::GUS reporter. j GUS activity at the base and placenta of WT flower at stage 12. k Staining in the early carpel development (k1), mature ovule (k2), and ectopic papilla (k3) in atring1a;atring1b mutant. l, m BP expression detected using BP::GUS reporter. l Staining at the base and placenta of WT flower. m Staining in the early carpel (m1), mature ovule (m2), and ectopic carpel (m3) in atring1a;atring1b mutant. n, o KNAT2 expression detected using KNAT2::GUS reporter. n Staining at the base and placenta of WT flower. o Staining in the early carpel (o1), in the placenta of mature carpel (o2), and in the ectopic carpel (o3) in atring1a;atring1b mutant. Ca carpel, FM floral meristem, O ovule, P petal, St stamen. Bar = 100 μm, except 50 μm in b–e, h, i1 and i2, and 10 μm in i3 and i4