Fig. 7
From: Search strategy is regulated by somatostatin signaling and deep brain photoreceptors in zebrafish
Loss of somatostatinergic neurons and opn4a expressing deep brain photoreceptors potentiates local search. a R-turn lock index for otpa wildtype siblings and mutants during full-field illumination (base) and immediately following loss of illumination (T0). N = 25 larvae (sibs, base), 27 (sibs, T0), 47 (mutants, base), 38 (mutants, T0). b Representative path trajectories for an otpa mutant larva during 10-min recording periods during baseline (orange trace) and after loss of illumination (black trace). Arrowheads denote starting positions. Chamber: 200 × 200 mm. Scale bar 2 mm. Color represents fractal dimension. c Fractal dimension of path trajectories for otpa wildtype siblings (black circles, N = 29) and mutants (grey circles, N = 31). * P < 0.05 for mutants versus siblings. Dashed line shows mean for mutants under full-field illumination. d Schematic diagram showing neuronal cell types within Orthopedia expression domain. Labeled neuron types correspond to the neuronal markers with reduced expression in the otpa mutant background. e Mean fractal dimension during 3–5 min following loss of illumination. Control group was injected with sgRNA against GFP in Tg(vglut2a:EGFP) transgenic larvae. Controls N = 26; otpa N = 31; mi174 N = 13; trh N = 37; sst1.1  N = 22; opn4a N = 36; valopa N = 6. * P < 0.05 for mutant groups versus control group. f Representative path trajectories of individual larva for a 10 minute duration following loss of illumination. Top left: CRISPR injected control; Top right: trh; Bottom left: sst1.1; and Bottom right: opn4a. Arrowheads note starting position at time of light extinction. Chamber: 200 × 200 mm. Color represents fractal dimension, with scale as for (b)