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Fig. 5 | BMC Biology

Fig. 5

From: Amyloid-like aggregation of provasopressin in diabetes insipidus and secretory granule sorting

Fig. 5

Deletion analysis of the glycopeptide with respect to ER aggregation. a Several amyloid prediction programs (including Aggrescan [42], Amylpred [43], and FoldAmyloid [44]) pointed to the sequence 120–129 in the middle of the glycopeptide sequence as potentially amyloidogenic. In construct Pro1∆120-9, this sequence was deleted. In addition, the glycopeptide was gradually truncated from the C-terminus in Pro1∆gp10/20/30. b Formation of aggregates in transfected HN10 cells revealed no significant effect of deleting the central sequence, but a gradual reduction of aggregate formation upon C-terminal truncation. The mean and individual values of two to four independent transfections (as indicated), analyzing ~200 expressing cells per transfection, are shown

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