Fig. 6

V5xA mutation reduces ER aggregation while still permitting precursor folding and secretion. a The fraction of cells expressing 1–75, 1–75Pro1, or 1–75V5xA forming ER aggregates was quantified as before (mean and individual values of three independent transfections, analyzing ~100 expressing cells per transfection). All pairwise comparisons are significantly different according to an unpaired two-tailed t test (**p < 0.01; ***p < 0.001). b Transfected cells were lysed, separated by reducing SDS-gel electrophoresis, and subjected to immunoblot analysis to detect intracellularly accumulated provasopressin constructs. Molecular weight standards are indicated (in kilodaltons). c Transfected HN10 cells expressing the indicated provasopressin constructs were labeled with [³⁵S]methionine for 30 min and chased for an additional 2 h. From cell lysates and media, products were immunoprecipitated and analyzed by gel electrophoresis and autoradiography. The positions of full-size glycosylated (FLg) and unglycosylated forms (FLu) and of constructs without glycopeptide (∆gp) are indicated