Fig. 2
From: Structural basis for potency differences between GDF8 and GDF11
Inhibition of GDF8 and GDF11 by known antagonists. a, b, c, d Luciferase reporter assays showing the inhibitory activity following titration of FS288 (a), FSTL3 (b), GASP1 (c), and GASP2 (d) against a constant concentration (0.62 nM, dashed bar) of GDF8 (blue) or GDF11 (orange) in HEK293 (CAGA)12 cells. Refer to Table 1 for a corresponding analysis of the inhibition curves. Data information: In a–d, data are presented as fraction activation (ligand response at antagonist concentration/ligand response at 0 nM antagonist). Each concentration was performed in triplicate and shown as the mean ± SEM of two to three independent experiments. Data from independent experiments were combined and fit to non-linear regression with a variable slope. Ligand source: gift from Acceleron Pharma