Structural basis for potency differences between GDF8 and GDF11

Table 1 Analysis of known antagonists to GDF8 and GDF11 by luciferase reporter assay

Ligand	Antagonist	IC₅₀ (nM)	IC₅₀ (nM) 95% CI^a	Log[IC₅₀(M)] ± SEM^b	Hill slope	Hill slope 95% CI
GDF8	FS288	0.2	0.21 to 0.23	−9.7 ± 0.01	−4.31	−5.17 to −3.43
GDF11	FS288	0.3	0.27 to 0.34	−9.5 ± 0.03	−4.77	−6.73 to −2.81
GDF8	FSTL3	1.2	1.08 to 1.30	−8.9 ± 0.02	−1.93	−2.19 to −1.68
GDF11	FSTL3	4.0	3.72 to 4.35	−8.4 ± 0.02	−5.41	−6.79 to −4.02
GDF8	GASP1	0.2	0.25 to 0.24	−9.6 ± 0.01	−4.47	−4.90 to −4.03
GDF11	GASP1	0.3	0.29 to 0.39	−9.1 ± 0.02	−4.34	−5.49 to −3.20
GDF8	GASP2	4.1	3.31 to 5.07	−8.4 ± 0.05	−1.64	−2.15 to −1.14
GDF11	GASP2	18.3	14.98 to 22.46	−7.7 ± 0.04	−1.67	−2.18 to −1.16

ISSN: 1741-7007