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Fig. 1 | BMC Biology

Fig. 1

From: Functional studies of Drosophila zinc transporters reveal the mechanism for zinc excretion in Malpighian tubules

Fig. 1

Tubule-specific modulation of Zip71B and ZnT35C expressions modifies flies’ zinc sensitivity. A Expression pattern of NP1093-GAL4 > UAS-GFP in the gut of third-instar larvae. NP1093-GAL4 is expressed specifically in the Malpighian tubules. Arrows denote the Malpighian tubules. Scale bars = 100 μm. B, C RT-PCR analysis of the knockdown effect of Zip71B-RNAi and ZnT35C-RNAi flies. rp49 was used as internal control. D, E Survival rate of tubule-specific Zip71B-RNAi and ZnT35C-RNAi flies under different food conditions. Both Zip71B-RNAi and ZnT35C-RNAi flies show increased sensitivity toward zinc (means ± SEM, n = 6, P < 0.001; two-tailed Student’s t test), but not other metals or metal chelator EDTA. Genotypes of the flies are NP1093-GAL4/+ for control, Zip71B-RNAi/+; NP1093-GAL4/+ for Zip71B-RNAi, and ZnT35C-RNAi/+; NP1093-GAL4/+ for ZnT35C-RNAi. F (a) RT-PCR analysis of the overexpression effect of Zip71B-OE and ZnT35C-OE flies. rp49 was used as internal control. (b) Statistical analysis of RT-PCR result in (a); n = 3. Genotypes of the flies are NP1093-GAL4/+ for control, UAS-Zip71B/+; NP1093-GAL4/+ for Zip71B-OE, and UAS-ZnT35C/+; NP1093-GAL4/+ for ZnT35C-OE. G Tubule-specific over-expression of Zip71B and ZnT35C confers resistance to high dietary zinc. While both Zip71B-OE and ZnT35C-OE flies showed resistance to high dietary zinc, Zip71B-OE and ZnT35C-OE flies displayed a synergistic effect. Data are presented as means ± SEM, n = 6. * P < 0.05, *** P < 0.001; two-tailed Student’s t test. Genotypes of the flies are NP1093-GAL4/+ for control, UAS-Zip71B/+; NP1093-GAL4/+ for Zip71B-OE, UAS-ZnT35C/+; NP1093-GAL4/+ for ZnT35C-OE, and UAS-ZnT35C/+; NP1093-GAL4/+ for ZnT35C-OE

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