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Fig. 2 | BMC Biology

Fig. 2

From: Functional studies of Drosophila zinc transporters reveal the mechanism for zinc excretion in Malpighian tubules

Fig. 2

Zinc is accumulated in the body of Zip71B-RNAi fly. a, b Metal contents in Zip71B-RNAi larvae on normal and 1 mM zinc-supplemented food. Tubule-specific RNAi of Zip71B led to a significant increase in whole-body zinc content but no other metal content in both normal food and 1 mM zinc food. Metal content values represent three independent ICP-MS measurements and are normalized to dry body weight; data are presented as means ± SEM, n = 3. *** P < 0.001; two-tailed Student’s t test. c Real-time analysis showing up-regulation of MtnB in Zip71B-RNAi larvae on normal and 1 mM zinc-supplemented food. rp49 was used as the reference gene for normalization. MtnB expression was shown as ratios relative to that of wild-type larvae raised on normal food. Values are presented as means ± SEM; n = 3. ** P < 0.01, *** P < 0.001, two-tailed Student’s t test. Genotypes of the flies are NP1093-GAL4/+ for control and Zip71B-RNAi/+; NP1093-GAL4/+ for Zip71B-RNAi

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