Fig. 8

Delta cell differentiation is disrupted in the cdx4 mutant embryos. Analysis of wild-type and cdx4 ^tv205–/– mutant embryos at 48 hpf by WHISH (a) and FISH (b–d) using delta cell markers (sst2, lamc2, slc7a7, and map3k15) as well as gcga and insulin. cdx4 ^tv205 mutants display a loss of sst2 (a), lamc2 (b), and slc7a7 (c) pancreatic expression and an increase of beta cells. Map3k15 expression is strongly reduced in the pancreatic islet (yellow arrows) while not affected in the presumptive pronephric glomeruli (white arrows) (d). No obvious effect is observed on gcga expression. Nuclei are stained with DAPI (grey staining). (Analyzed embryos > 10)