Fig. 1

A novel end-to-end Multi-Animal Tracker software allows recording, tracking, and analyzing multiple animals at a time. a Flowchart describing the sequential usage of the different packages included in the software suite. b A simple, user-friendly Graphical User Interface is provided to analyze tracks of multiple animals throughout a movie recording typically consisting of thousands of frames. The tracking software uses a machine learning algorithm to identify animal instances. The user is asked to ‘teach’ the software what is an animal by clicking on several animal instances. c–f The software is able to track and analyze the movement of different animals. Shown are representative tracks extracted from movies of behaving (c) worms, (d) flies, (e) zebrafish, and (f) mice