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Fig. 3 | BMC Biology

Fig. 3

From: OsLG3 contributing to rice grain length and yield was mined by Ho-LAMap

Fig. 3

OsLG3 is a nuclear-localized transcriptional activator. a Subcellular localization of OsLG3. b Transactivation assay of truncated OsLG3. Fusion proteins of the GAL4 DNA-binding domain and different portions of OsLG3 were expressed in yeast strain AH109. A1 indicates the full-length coding sequence of OsLG3; A2 to A5 indicate the mutated forms of OsLG3 (nucleotide positions were labeled in the diagrams), respectively. P and N indicate the positive and negative control, respectively. The culture solution of the transformed yeast was dropped onto the control plate SD/-Trp or selective plate (SD/-Trp/-Ade/-His/X-α-gal). The plates were incubated for 3 days

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