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Fig. 11 | BMC Biology

Fig. 11

From: Cleavage modification did not alter blastomere fates during bryozoan evolution

Fig. 11

Gene expression details with cell resolution in M. membranacea. Selected embryos from the in situ hybridizations shown in Figs. 9 and 10 observed under a compound microscope with a fluorescent lamp (green = nuclei, magenta = signal) or maximum intensity projections from confocal microscopy (gray = nuclei, magenta = signal). Arrowheads point to the relevant areas of gene expression while dashed areas mark unspecific background staining. Asterisks mark the position of the blastopore. a Expression of six3/6 at different focal levels. b Expression of otx in a vegetal view (left) showing the posterior gap in expression (triangle outline), neural cells at the apical disc (nc) of a mid gastrula embryo and the wider expression in the late gastrula (arrowheads). c Expression of foxa at 9.7 hpa in the 90-cell stage (left) without signal on the posterior cells 2dR and 3d, and the same posterior gap one cell division cycle later (right). d Late gastrula stage (left) viewed from the posterior vegetal end to show the mouth opening with surrounding expression of foxa (B quadrant is bottom). On the right, a left side view with foxa expression in the mouth region. e Bilateral anterior cells expressing gsc. f Two nanos-positive cells during mid gastrula (left) and late gastrula (right). g Posterior and lateral cells on the vegetal ectoderm expressing bra (left) and a posterior view of a late gastrula depicting the domain in the posterior epithelium of the vestibule (right). h Vegetal view of early gastrula with the two vegetal cells with cdx expression (2dR21 and 2dR22). i cdx expression observed in two cells at the posterior ectoderm (left), and in the 4d cell (right) at mid gastrula with two. j Expression of evx in one posterior ectodermal cell (2dR2) on the vegetal side during early gastrulation (left). Progeny of 2dR2 expresses evx (center) as well as the derivatives of 4a and 4c and the 4d cell. k Mid gastrula stage with evx expression in at least two posterior ectodermal cells (left) and in the 4d (right). l Posterior view of a late gastrula with evx expressed in the posterior endoderm (ed) and ectoderm (ec). m Left side view of evx expression at the late gastrula with posterior endodermal and ectodermal domains. n Expression of wnt1 during early gastrulation is restricted to three cells, 2dL2, 2dR2, and 3d2 (left) and a posterior cluster of cells at the late gastrula (right). o twist expression in internalized blastomeres. p Expression of gata456b from 32-cell stage until early gastrulation. Transcripts are restricted to the 3D until the internalization of vegetal blastomeres, when 4A and 4C initiate the expression of gata456b. Scale bar = 10 μm

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