Fig. 8From: Cleavage modification did not alter blastomere fates during bryozoan evolutionMAPK activity during the development of M. membranacea. Confocal maximum intensity projections of embryos incubated with the antibody against the activated form of MAPK (Diphosphorylated ERK-1&2) (yellow) and counterstained with Sytox Green for nuclei (green). a No detectable levels of activated MAPK at the 16-cell stage (5.2 hpa). b 28-cell stage in vegetal view at 6.7 hpa showing the first detectable MAPK activity. c Side view at 7.5 hpa showing the quadrants A and D of a 32-cell stage with activated MAPK in the cell 3D. d 40-cell stage at 8 hpa. e Vegetal view of a 48-cell stage at 9 hpa with blastomeres 3C and 3A undergoing mitosis. f Frontal view of an embryo with approximately 90 cells at 9.7 hpa. 3D cell shows a weaker signal for MAPK activity. g Embryo soon after the division of the 3D cell at 13.1 hpa. There are no detectable levels of MAPK activity in any cell. h Embryo with more than 125 cells (16 hpa) without any detectable levels of MAPK activity. Scale bar = 10 μmBack to article page