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Fig. 8 | BMC Biology

Fig. 8

From: Cleavage modification did not alter blastomere fates during bryozoan evolution

Fig. 8

MAPK activity during the development of M. membranacea. Confocal maximum intensity projections of embryos incubated with the antibody against the activated form of MAPK (Diphosphorylated ERK-1&2) (yellow) and counterstained with Sytox Green for nuclei (green). a No detectable levels of activated MAPK at the 16-cell stage (5.2 hpa). b 28-cell stage in vegetal view at 6.7 hpa showing the first detectable MAPK activity. c Side view at 7.5 hpa showing the quadrants A and D of a 32-cell stage with activated MAPK in the cell 3D. d 40-cell stage at 8 hpa. e Vegetal view of a 48-cell stage at 9 hpa with blastomeres 3C and 3A undergoing mitosis. f Frontal view of an embryo with approximately 90 cells at 9.7 hpa. 3D cell shows a weaker signal for MAPK activity. g Embryo soon after the division of the 3D cell at 13.1 hpa. There are no detectable levels of MAPK activity in any cell. h Embryo with more than 125 cells (16 hpa) without any detectable levels of MAPK activity. Scale bar = 10 μm

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