Fig. 6

Hybrid does not slow-down its growth during ethanol stress. a, b A minority of hemizygote strains overtakes the S. cerevisiae pool when subject to ethanol stress. a Number of strains that were reliably identified (>30 reads) when sequencing the hemizygote hybrid or S. cerevisiae pools at different time points in YPD + 8% ethanol medium. b Relative representation of each strain within the S. cerevisiae pool. Red points represent the strains that were abundant (log₂(TPM + 1) > 2) at the last two time points in both S. cerevisiae experiments. c Strains deleted for genes coding for ribosomal proteins are enriched in the survivor pool. Shown is the hypergeometric P value for enrichment of ribosomal genes. d Increasing retrograde signaling improves hybrid growth under ethanol stress. Cultures were diluted periodically to maintain the cells in logarithmic growth. MKS1 is a negative regulator, whereas RTG2 is an activator of retrograde pathway. ΔΔ refers to strains deleted in both alleles of the gene