Fig. 2
From: Central peptidergic modulation of peripheral olfactory responses
NPF-NPFR signaling sensitizes ab3A neurons. a Peak odor-evoked ab3A activity from 386Y > NPF-IR flies and heterozygous controls responding to solvent (paraffin oil, PO) or ethyl butyrate (EB 10−6 or 10−5 v/v). Replicates: 386Y-GAL4/+, PO = 14; EB 10−6 and 10−5 = 6; UAS-NPF-IR/+, PO = 25, EB 10−6 = 13, EB 10−5 = 12; 386Y > NPF-IR, PO = 9, EB 10−6 and 10−5 = 6. b Peak odor-evoked ab3A activity from NPF > NPF-IR flies and heterozygous controls responding to PO or EB 10−6 or 10−5 v/v. NPF-GAL4/+, PO = 15; EB 10−6 and 10−5 = 6; UAS-NPF-IR/+, PO = 15, EB 10−6 and 10−5 = 6; NPF > NPF-IR, PO = 12, EB 10−6 and 10−5 = 6. c Spontaneous ab3A activity from NPF > NPF-IR flies and heterozygous controls. Boxplot whiskers indicate minimum and maximum values, n = 12, one-way ANOVA, non-significant (ns). d Peak odor-evoked ab1A/B activity from NPF > NPF-IR flies and heterozygous controls responding to PO or EB 10−5 or 10−4 v/v. Replicates for all genotypes: PO = 12, EB 10−5 and 10−4 = 6. e Peak odor-evoked ab2A activity from NPF > NPF-IR flies and heterozygous controls responding to PO or methyl acetate (MA 10−7 or 10−6 v/v). Replicates for all genotypes: PO = 12, EB 10−7 and 10−6 = 6. f Peak odor-evoked ab3A activity from w 1118, homozygous NPFR c01896, transheterozygous NPFR c01896 /NPFR Def, and heterozygous controls responding to PO or ethyl butyrate (EB 10−6 or 10−5 v/v). Replicates: w 1118, PO = 28, EB 10−6 and 10−5 = 14; NPFR c01896, PO = 26, EB 10−6 and 10−5 = 12; NPFR Def, PO = 15, EB 10−6 and 10−5 = 6; NPFR c01896 /+, PO = 17, EB 10−6 and 10−5 = 6; NPFR c01896 /NPFR Def, PO = 15, EB 10−6 and 10−5 = 6. The data in a–f represent recordings from 7- to 14-day-old flies of both sexes (50:50). All heterozygous controls were crossed to w1118. The data in a, b, and d–f are presented as means ± 95% confidence intervals, two-way ANOVA, non-significant (ns), P ≤ 0.01 (**), P ≤ 0.001 (***)