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Fig. 4 | BMC Biology

Fig. 4

From: Central peptidergic modulation of peripheral olfactory responses

Fig. 4

ab3A neurons express NPFR. a Peak odor-evoked ab3A activity from Orco > NPF-IR flies and heterozygous controls responding to solvent (paraffin oil, PO) or ethyl butyrate (EB 10−6 or 10−5 v/v). Replicates: Orco-GAL4/+, n = 6; UAS-NPFR-IR/+, PO = 8, EB 10−6 and 10−5 = 6; Orco > NPFR-IR, n = 6. b Peak odor-evoked ab3A activity from Or22a > NPF-IR flies and heterozygous controls responding to PO or EB 10−6 or 10−5 v/v. Replicates: Or22a-GAL4/+, PO = 12, EB 10−6 and 10−5 = 6; UAS-NPFR-IR/+, PO = 16, EB 10−6 and 10−5 = 10; Or22a > NPFR-IR, PO = 12, EB 10−6 and 10−5 = 6. The data in a and b are presented as means ± 95% confidence intervals, two-way ANOVA, P ≤ 0.001 (***). The heterozygous controls in a and b were crossed to w1118. c Sample traces for ab3 sensilla from the indicated genotypes responding to a 0.5-s stimulation (black bar) of EB (10−6 v/v). d A quantification of the results presented in c. Recordings were performed on 7- to 14-day-old flies of both sexes. Ab3A neuron-specific expression of NPFR-RD but not NPFR-RB using Or22a-GAL4 rescues the reduced EB responsiveness of the NPFR c01896 mutant. The data in d are presented as means ± 95% confidence intervals, one-way ANOVA, P ≤ 0.001 (***). Replicate numbers for w 1118 = 14; NPFR c01896 = 12; Or22a > NPFR-RD, NPFR c01896 = 12; UAS-NPFR-RD, NPFR c01896 = 12; Or22a-GAL4, NPFR c01896 = 14; UAS-NPFR-RB, NPFR c01896 = 12; Or22a > NPFR-RB, NPFR c01896 = 12

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