Fig. 6

NPFR may affect OR22a localization but not expression. a A schematic showing the ab3A neurons’ soma, inner dendrites, ciliary dilations, and outer dendrites. Wild-type OR22a staining is indicated in yellow. b, c Representative images of ab3A neurons from w ¹¹¹⁸ (center) and NPFR ^c01896 (right) stained with an OR22a protein-specific antiserum. Some of the ciliary dilations are marked by open triangles. The color in these images is inverted to improve visibility. Scale bar, 5 μm. d Cropping only the outer dendritic segments as regions of interest (yellow boxes) and quantifying and selecting the pixels with intensity scores >50 using ImageJ, we found no difference between w ¹¹¹⁸ and NPFR ^c01896 ab3A neurons in OR22a staining. Boxplot whiskers indicate minimum and maximum values, n = 19 sensilla per genotype, Student’s t test, non-significant (ns). e By tightly cropping the puncta with the strongest signal intensity in each section, we found a significant increase in punctal staining near the ciliary dilations of NPFR ^c01896 antennae as compared to w ¹¹¹⁸ antennae. Boxplot whiskers indicate minimum and maximum values, Student’s t test, P ≤ 0.001 (***). Replicate numbers for w ¹¹¹⁸ = 11; NPFR ^c01896 = 14 puncta. f qPCR results show no significant difference in Or22a mRNA expression between w ¹¹¹⁸ and NPFR ^c01896 antennae of mixed sexes. Data are presented as means ± 95% confidence intervals, n = 4, Student’s t test, non-significant (ns)