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Fig. 1 | BMC Biology

Fig. 1

From: Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues

Fig. 1

Validation of anti-mouse DARC MAb reactivity. a Representative flow cytometry histograms of control HEK-293 cells (none) and HEK-293 cells transfected with DARC or DARC-eGFP fusion protein stained with anti-mouse DARC MAb (n = 3 experiments). b Flow cytometry analysis of anti-mouse DARC MAb reactivity with RBCs. Peripheral blood was harvested from wildtype (WT) and DARC–/– mice and stained with a MAb against the RBC marker TER-119 and anti-mouse DARC MAb (black line) or isotype control MAb (grey) (n > 8 experiments). c Western blot analysis of anti-mouse DARC MAb reactivity with protein lysates from untransfected HEK-293 cells and HEK-293 cells stably transfected with DARC or DARC-eGFP fusion protein. b-Actin is shown as loading control (n = 4 experiments). d Representative confocal fluorescent micrographs of frozen sections of peripheral lymph nodes from DARC WT and DARC–/– mice stained with MAbs against CD31 (green), peripheral node addressin (PNAd; blue), and DARC or isotype control (red). 20× objective, scale bars = 200 μm (n = 3 experiments)

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