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Fig. 2 | BMC Biology

Fig. 2

From: Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues

Fig. 2

DARC is a robust marker of venular endothelial cell differentiation. a Representative confocal micrographs of whole mount stainings of adipose tissue (omentum, 20× objective, scale bars = 200 μm, top row) and skin (ear, 10× objective, scale bars = 300 μm, bottom row) with MAbs against CD31 (green), DARC (red), and LYVE-1 (blue). Arrows in images on the left identify representative arterioles (art.), capillaries (cap.), and venules (ven.). The boxed area in the merged image of adipose tissue was magnified (far right, top row) to illustrate concentrated DARC staining at inter-endothelial junctions (arrowhead). Adipose tissue n > 10 experiments, skin n > 10 experiments. b Semi-quantitative analysis of DARC expression in arterioles (art.), capillaries (cap.), pre-venular capillaries (PVC), post-capillary venules (PCV), and collecting venules (col. ven.) in adipose tissue. Data represent 16 microvascular trees from seven fields of view of omenta from two mice. Supporting data values are included in Additional file 2. Representative confocal micrographs of tissues stained with anti-CD31 (green) and anti-DARC (red) depict positive, partial, and negative DARC staining on PVCs and partial DARC expression on PCVs. Error bars depict mean ± SEM

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