Fig. 3

DARC expression on whole mount and frozen section stainings. Representative confocal micrographs of whole mount staining of (a) cremaster muscle (20× objective, scale bar = 200 μm), (b) bladder (10× objective, scale bar = 300 μm), and (c) conjunctival vessels (10× objective, scale bar = 300 μm) with MAbs against CD31 (green) and DARC (red). Representative confocal micrographs of frozen sections from (d) brain (20× objective, scale bar = 200 μm), (e) Peyer’s patches (10× objective, scale bar = 300 μm), (f) mesenteric lymph nodes (20× objective, scale bar = 200 μm), (g) thymus (10× objective, scale bar = 300 μm), (h) pancreas (20× objective, scale bar = 200 μm), (i) small intestine (20× objective, scale bar = 100 μm), and (j) colon (10× objective, scale bar = 300 μm) with MAbs against: CD31 (green), DARC (red), insulin (white), MAdCAM1 (white), or UEA-1 (white) as indicated. L indicates lumen of small intestine and colon. n = at least three experiments for each tissue