Fig. 4

Identification of DARC⁺ venular endothelial cells (V-ECs) by flow cytometry. a Gating strategy to identify EC subsets in single cell suspensions of murine lymph nodes; BEC blood ECs, LEC lymphatic ECs, NV-EC non-venular EC. b Using the same gating strategy as in panel A, the frequency of DARC⁺ V-EC and DARC^– NV-EV among BECs was assessed in multiple tissues (upper row). The lower row shows the same samples stained with an isotype-matched control MAb. c Quantitative assessment of the frequency of DARC⁺ V-ECs among total BECs in a panel of fresh murine tissues. Error bars depict mean ± SEM of recovered V-EC frequencies among BECs in mesenteric LN (n = 11), peripheral LN (n = 14), skin (n = 18), adipose tissue (n = 6), colon (n = 10), small intestine (n = 6), brain (n = 5), and pancreas (n = 5). ^ns P > 0.05, *P ≤ 0.05; **P ≤ 0.01