Fig. 1

Generation of keratin-5-matriptase zymogen-locked and keratin-5-matriptase catalytically inactive transgenic mice. a Schematic structure of the keratin-5-matriptase transgenes. The bovine keratin-5 promoter (K5), rabbit β-globin exons, rabbit β-globin intron B, mouse matriptase cDNA, and rabbit β-globin polyadenylation signal (Poly A) are shown. b Expression of the keratin-5-matriptase transgenes in mouse skin. RT-PCR analysis of skin from an established keratin-5-matriptase zymogen (K5R614Q, lanes 1 and 2) transgenic mouse line, two wildtype littermates (WT, lanes 3 and 4), an established keratin-5-matriptase catalytically inactive (K5S805A, lanes 5 and 6) transgenic mouse line, and two wildtype littermates (WT, lanes 7 and 8). c and d Quantitative real-time PCR analysis with primer pairs specific for transgenic matriptase mRNA (c) or total matriptase mRNA (d) in skin from newborn wildtype mice (WT, n = 3 mice), keratin-5-matriptase zymogen-locked transgenic (K5R614Q, n = 4 mice) littermates, wildtype mice (WT, n = 3 mice), and keratin-5-matriptase catalytically inactive transgenic (K5S805A, n = 3 mice) littermates. Horizontal bars are means of individual values. e Western blot analysis of total matriptase protein in skin from newborn wildtype mice (lanes 1–4) and keratin-5-matriptase zymogen-locked (K5R614Q, lanes 5–8) littermates. Quantification of band intensity from scanned Western blot from (e) is shown on the right (mean ± SD). f Western blot analysis of matriptase in skin from newborn wildtype mice (lanes 1–3) and keratin-5-matriptase catalytically inactive (K5S805A, lanes 4–7) littermates. Matriptase null skin was included as negative control (KO, lane 8). Molecular weight markers are shown on the left. Quantification of band intensity from scanned Western blot from F is shown on the right (mean ± SD). P values were determined by Student’s t test. Additional file 1: Raw supporting data