Fig. 2

Promuscular col expression does not impact fusion competent myoblast (FCM) fate. Stage 15 col ^ECRM -H2B-RFP (A) and col ¹ ,col ^ECRM -H2B-RFP (B) embryos stained for RFP (red) and β3-tub (green) to visualise the nuclei of Col⁺ PMC myoblasts and all muscles, respectively; (A’, B’) red channel only; (A”, B”) close up of three abdominal hemisegments. C Number of RFP⁺ nuclei in DA2, DA3 and DT1 at stage 15 in col ^ECRM -H2bRFP and DA2, DA3 > DA2 and DT1 in col ¹ ,col ^ECRM -H2bRFP embryos. The DA3 transformation into a DA2-like muscle (DA3 > DA2) in col ¹ embryos is schematised on the right. D FISH of nascent col transcripts (green) in stage 14 col ^ECRM -H2bRFP embryos stained for RFP (red) and Col (blue); col is transcribed in a fraction of both RFP⁺ and RFP^– DA3 nuclei. Scale bar: 20 μm