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Fig. 5 | BMC Biology

Fig. 5

From: Dynamics of transcriptional (re)-programming of syncytial nuclei in developing muscles

Fig. 5

Independent transcription of identity transcription factors and generic muscle differentiation genes. A–E wt embryos at various embryonic stages, stained for Mef2 (red) and Col (blue) coupled to FISH against nascent Mhc transcripts. The white arrow points to Mhc expression in heart cells, first detected at stage 15. F Double FISH against nascent col (red) and Mhc (green) transcripts, and Col staining (blue) to visualise the DA3 nuclei at stage 15. F’ same as (F), DAPI staining (blue) shows all nuclei. F” Col staining only. Single Z sections. The yellow arrow indicates a nucleus with low Col protein, which transcribes Mhc. G Box plots showing the number of nuclei transcribing either col, Mhc or both, relative to the total number of DA3 nuclei

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