Fig. 6

Transcription dynamics of identity-realisation genes. A, E, I, M, Q Stage 14 col ^LCRM -moeGFP; S59-mcd8GFP embryos stained for GFP (green) and Topro (blue) to visualise DA3 and DT1 and all nuclei, respectively, coupled with FISH of either nascent duf (A), Pax (E), kon (I), mspo (M) or Con (Q) transcripts (red); A’, E’, I’, M’, Q’ Red channel only, muscle contours are outlined by dashed grey lines. B, F, J, N, R Number of transcription dots in DA3 and DT1 at different embryonic stages. Bar graphs indicate the mean number of nuclei; error bars, the SD. C, G, K, O, S Intensity (IntDen) of transcription dots in DA3 and DT1; each dot is represented by a circle, the bar graphs show the mean value and SD. D, H, L, P, T Cumulative intensity of transcriptional dots (Total IntDen per fibre) in DA3 and DT1. The mean value is shown; error bars correspond to the SEM