Fig. 2

Representative FRET efficiency histograms obtained for different time points during the first 30 h of aggregation, recorded upon dilution into deionized water for sm-FRET detection. Here, the histograms have been split into two populations of apparent sizes, small-mers (2–5 monomer units) and large-mers (6–150 monomer units). For large-mers, the differences in the FRET efficiency histograms are clearly observable, especially at times longer than 24 h. In the histograms of ɑS-only and ɑS with NbHul5g, the peak centered at the FRET efficiency value of 0.8 is dominant and corresponds to high-FRET oligomers. In the presence of both ɑS-specific nanobodies, the histograms are centered at the FRET efficiency value of 0.5, and the maximum at 0.8 is absent