Fig. 4
From: Silicanin-1 is a conserved diatom membrane protein involved in silica biomineralization
Localization of Sin1-GFPC around the time of valve biogenesis. a Schematic drawings illustrating the different stages of the diatom cell cycle. For simplicity, intracellular compartments, except for the SDVs, have been omitted. Black and blue colors indicate mature biosilica and newly produced biosilica, respectively. Red and yellow colors depict the plasma and SDV membranes, respectively. b–d Selected images from time-lapse confocal fluorescence microscopy of Sin1-GFPC labeled with PDMPO are shown (Additional file 2: Movie S1). The time above the images relates to the peak of the GFP and PDMPO fluorescence (Fig. 5), which is set as t = 0 min. Panel b shows the GFP fluorescence (green), panel c the PDMPO fluorescence (blue), and panel d an overlay of GFP and PDMPO fluorescence (note: a superposition of green and blue fluorescence appears cyan). All images are projections of nine z-planes. Scale bars: 2 μm