Fig. 5

Time-dependent quantitative analysis of region-specific (a) GFP and (b) PDMPO fluorescence during valve formation in Sin1-GFP^C expressing cells. The plots show the averaged results from four different transformant cells labeled with PDMPO. From each cell, images were recorded in 3.5 min intervals, and the fluorescence intensities in different regions of the cell were determined. From each frame, z-projections were generated by combining all nine z-planes. The schematic shows the delineations of the cellular regions analyzed. The coloring of the cellular regions in the schematic corresponds to the line coloring in the graphs. The black lines represent the sum of the intensities from all regions of the cell. The frame with the maximum GFP and PDMPO fluorescence during the time-lapse recording was defined as t = 0 min. This allowed alignment of the time scale of the four different cells used in our analysis. The gray-shaded areas represent the standard deviation of the averaged fluorescence intensities obtained from the four cells for each region. No standard deviation is given for time periods for which only a single cell was available for averaging