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Table 1 Acidic residues are rarer in TMHs of single-pass proteins than in TMHs of multi-pass proteins

From: Charged residues next to transmembrane regions revisited: “Positive-inside rule” is complemented by the “negative inside depletion/outside enrichment rule”

Dataset Acidic residues (D and E) Aspartic acid (D only) Glutamic acid (E only)
μ SP μ MP H statistic P value μ SP μ MP H statistic P value μ SP μ MP H statistic P value
ExpAll 0.086 0.309 148.1 4.50E-34 0.045 0.157 40.3 2.13E-10 0.042 0.161 46.6 8.64E-12
UniHuman 0.076 0.398 316.5 8.31E-71 0.034 0.191 91.6 1.05E-21 0.042 0.207 100.3 1.33E-23
UniER 0.106 0.430 34.4 4.39E-9 0.061 0.161 8.0 4.72E-3 0.045 0.268 26.8 2.24E-7
UniGolgi 0.097 0.381 39.8 2.88E-10 0.043 0.180 19.4 1.05E-5 0.053 0.201 20.2 7.01E-6
UniPM 0.039 0.400 121.0 3.86E-28 0.016 0.187 32.7 1.06E-8 0.022 0.213 36.9 1.26E-9
UniCress 0.062 0.434 163.5 1.99E-37 0.036 0.198 32.5 1.20E-8 0.025 0.241 66.0 4.59E-16
UniFungi 0.177 0.349 43.1 5.14E-11 0.044 0.166 24.5 7.60E-7 0.133 0.183 4.6 0.033
UniBacilli 0.089 0.352 24.1 9.16E-7 0.048 0.185 11.2 8.27E-4 0.040 0.176 12.3 4.54E-5
UniEcoli 0.148 0.315 2.7 0.100 0.111 0.150 0.1 0.729 0.037 0.163 2.2 0.140
UniArch 0.438 0.606 1.8 0.183 0.083 0.344 11.2 8.33E-4 0.354 0.247 3.5 0.0624
  1. The statistical results when comparing the number of acidic residues in single-pass or multi-pass TMHs within their database-defined limits and excluding any flanks. The number of helices per dataset can be found in Table 2 for single-pass TMHs and Table 3 for multi-pass helices. µSP is the average number of the respective residues per helix in TMHs from single-pass proteins, while µMP is the average number of the respective residues per TMH from multi-pass proteins. The Kruskal-Wallis test scores (H statistics) were calculated for the numbers of aspartic acid and glutamic acid residues in each helix from single-pass and the number of aspartic acid and glutamic acid residues in each helix from multi-pass TMHs
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