Single-pass | Database-defined flanks | Flanks after central alignment |
---|
Dataset | Helices | Negative residues |
H statistic |
P value | Negative residues |
H statistic |
P value |
---|
| | Inside | Outside | | | Inside | Outside | | |
---|
ExpAll | 1544 | 848 | 1648 | 258.59 | 3.47E-58 | 735 | 1541 | 262.29 | 5.44E-59 |
UniHuman | 1705 | 780 | 1922 | 421.53 | 1.13E-93 | 652 | 1865 | 501.86 | 3.74E-111 |
UniER | 132 | 78 | 156 | 23.76 | 1.09E-06 | 76 | 150 | 21.62 | 3.33E-06 |
UniGolgi | 206 | 60 | 240 | 104.45 | 1.61E-24 | 54 | 239 | 107.18 | 4.06E-25 |
UniPM | 493 | 197 | 578 | 177.68 | 1.56E-40 | 161 | 569 | 215.18 | 1.02E-48 |
UniCress | 632 | 314 | 450 | 18.23 | 1.96E-05 | 231 | 444 | 55.80 | 8.01E-14 |
UniFungi | 729 | 449 | 631 | 28.15 | 1.12E-07 | 413 | 627 | 38.08 | 6.79E-10 |
UniBacilli | 124 | 90 | 113 | 3.73 | 5.35E-02 | 86 | 106 | 2.53 | 1.12E-01 |
UniEcoli | 54 | 32 | 77 | 17.24 | 3.30E-05 | 30 | 74 | 14.74 | 1.24E-04 |
UniArch | 48 | 113 | 8 | 49.66 | 1.83E-12 | 96 | 7 | 45.62 | 1.43E-11 |
- The “Helices” column refers to the total TMHs contained in each dataset (ExpALL, TMHs from TOPDB [50]; UniHuman, human representative proteome; UniER, human endoplasmic reticulum representative proteome; UniGolgi, human Golgi representative proteome; UniPM, human plasma membrane representative proteome; UniCress, Arabidopsis thaliana (mouse-ear cress) representative proteome; UniFungi, fungal representative proteome; UniBacilli, Bacilli class representative proteome; UniEcoli, Escherichia coli representative proteome; UniArch, Archaea representative proteome; see Methods for details). In the “Database-defined flanks” column, the “Negative residues” column refers to the total number of negative residues found in the ±10 flanking residues on either side of the TMH and does not include residues found in the helix itself. In the “Flanks after central alignment” column, the “Negative residues” column refers to the total number of negative residues found in the –20 to –10 residues and the +10 to +20 residues from the centrally aligned residues of the TMH. Unlike the other tables, the global averages are derived from the ±20 datasets. The Kruskal-Wallis scores were calculated for negative residues by comparing the number of negatively charged residues that were within the 10 inside residues and the 10 outside residues in either case