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Fig. 3 | BMC Biology

Fig. 3

From: Distinct unfolded protein responses mitigate or mediate effects of nonlethal deprivation of C. elegans sleep in different tissues

Fig. 3

Worm sleep deprivation triggers the UPRmt. a Intestinal fluorescence of the ubl-5p::ubl-5::gfp UPRmt reporter before and after deprivation, mock deprivation, and stimulation of mid-L4 larvae. b Intestinal fluorescence of the mitochondrial chaperone hsp-6p::gfp reporter after deprivation and mock deprivation. Horizontal lines, boxes, and bars depict medians, 1st and 3rd quartiles, and 5th and 95th percentiles, respectively. Single and double asterisks denote significant differences with p < 0.05 and p < 0.01, respectively. c Relative expression of ubl-5 and hsp-6 in deprived as compared to undisrupted wild-type animals (2 biological replicates, 20 animals per sample). The mechanical stimulus was applied for 4 h (which included L4 lethargus), and RNA was prepared immediately after this period. Error bars depict mean ± SEM

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