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Fig. 7 | BMC Biology

Fig. 7

From: Distinct unfolded protein responses mitigate or mediate effects of nonlethal deprivation of C. elegans sleep in different tissues

Fig. 7

Worm sleep deprivation causes germ cell apoptosis. a Brood sizes of daf-16(mu86); ced-3(n1286) double mutants during the first 3 days after L4 lethargus (t = 0 is 10–12 h after the fourth molt). Deprivation failed to induce a reduction in brood size. The dotted line depicts the brood size of deprived daf-16(mu86) mutants at t = 60 h from Fig. 6a. b Box plots of ced-1::gfp fluorescence after deprivation (left) or mock deprivation (right), normalized by the mean pre-treatment fluorescence. Horizontal lines, boxes, and bars depict medians, 1st and 3rd quartiles, and 5th and 95th percentiles, respectively. Sample sizes are denoted in parentheses; double asterisks depict a significant difference (p < 0.01). c Left: the number of sperm detected in a single gonad per animal. Horizontal lines, boxes, and bars depict means, 95% confidence intervals, and standard deviations, respectively. Inset: a confocal image of adult hermaphrodite sperm nuclei, specifically labeled by GFP-histone fusion driven by the Pcomp-1 promoter. Right: the ratio between the sperm count of deprived and control animals. Error bars depict mean ± SEM. Sample sizes are denoted in parentheses; single and double asterisks depict significant differences (between the mean and 1 or between genotypes) with p < 0.05 or p < 0.01, respectively

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