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Fig. 1 | BMC Biology

Fig. 1

From: Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps

Fig. 1

Flies devoid of inducible TEPs are viable and do not show increased susceptibility to wounding. a Left panel: eGFP-TEP2 proteins produced by an endogenously eGFP-tagged TEP2 locus were detected in haemolymph samples using an anti-GFP antibody. UC unchallenged, Bb septic injury with B. bassiana, Ctrl haemolymph from control y,w flies (not expressing a GFP). Three bands corresponding in size to the full-length tagged protein and two products of proteolytic cleavage were observed (highlighted with *). Right panel: eGFP-TEP4 proteins produced in flies overexpressing a TEP4-GFP fusion using a fat body driver (genotype UAS-TEP4-GFP/+;; Lpp-Gal4/+). A major band with the expected size corresponding to eGFP-TEP4 was observed as well as many smaller bands. A shorter cleaved product was observed at 48 h post-infection. b Genomic location of the four genes encoding secreted TEPs with the position of the transposon insertions causing mutation. c Lifespan of unchallenged male and female flies at 25 °C. TEPq Δ flies have a shorter lifespan than the w 1118 controls (log-rank test, P < 0.001). d Survival to clean injury. Males were pricked in the thorax with a clean needle and kept at 25 °C. TEPq Δ flies are as resistant as the wild-type (log-rank test, P > 0.05). e Survival to oxidative stress. Flies were fed on 1.5% H2O2 in standard food and flipped on fresh medium every 2 days. TEPq Δ flies are as resistant as the wild-type (log-rank test, P > 0.05). c, d, e Shown are representative survival experiments of a minimum of two independent repeats. Forty flies minimum were used for each genotype per repeat

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