Fig. 3From: Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid waspsSurvival to fungal infection. Male flies were either covered with spores (a, b, labelled as natural infection) or pricked in the thorax with a needle dipped in a concentrated fungal spore suspension (c–f). Data were analyzed by log-rank test. Shown are representative experiments of a minimum of two independent repeats (three where a difference from the control flies was observed). x-axis: time post-infection in days; y-axis: percentage of living flies. a–c Statistically significant differences were observed between TEPq Δ and wild-type (w 1118) flies (P < 0.001 for B. bassiana, both natural infection and pricking; P = 0.0039 for M. anisopliae). d–f No statistically significant difference was observed between the TEPq Δ and the wild-type (w 1118) flies in the case of N. crassa, A. fumigatus and C. albicans infection by septic injury. g Quantification of B. bassiana DNA 3 days post-infection normalised to the host RpL32 DNA. Values represent the mean ± standard error (SE) of three independent experiments and were analysed using Mann-Whitney test (two-sided). The quantity of fungal DNA is significantly elevated in the TEPq Δ flies as compared to the control w 1118 line (P < 0.001). Overactivation of the Toll pathway in TEPq Δ flies by overexpressing ModSP rescues the increased fungal growth caused by the absence of TEPq Δ (P = 0.005)Back to article page