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Fig. 6 | BMC Biology

Fig. 6

From: Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps

Fig. 6

Toll and Imd pathway induction in the TEPq Δ mutant. Expression of antimicrobial peptide genes normalised to ribosomal protein gene RpL32 after septic injury. a Induction of Diptericin (Dpt, Imd pathway read-out) in response to septic injury with E. carotovora. TEPq Δ flies show a wild-type level of induction of Diptericin. b Induction of Drosomycin (Drs; Toll pathway read-out) in response to septic injury with M. luteus. TEPq Δ flies show a significantly lower level of Drs expression 24 h post-infection (P = 0.013). c TEPq Δ flies show a significantly lower level of Drs expression 24 h post-infection with E. faecalis (P < 0.001 at 48 h post-infection). d TEPq Δ flies show a reduced Drs expression after septic injury with B. bassiana (P = 0.005 at 24 h post-infection and P < 0.001 at 48 h post-infection). e TEPq Δ flies show reduced Drs expression in response to the injection of purified E. faecalis peptidoglycan (PG; measured 16 h post-injection, P = 0.0286, Mann-Whitney test, two-sided) and heat inactivated spores of B. bassiana (Heat inactivated; measured 16 h post-injection, P = 0.0079, Mann-Whitney test, two-sided). f Drosomycin expression 24 h after challenge with B. bassiana is enhanced in TEP4-GFP overexpressing flies (P = 0.0079). UC unchallenged; Sec-GFP, flies overexpressing a secreted form of GFP were used as control. Data were analysed using t test comparing the values in TEPq Δ flies to wild-type w 1118 flies. Values represent the mean ± SE of at least two independent experiments

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