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Fig. 7 | BMC Biology

Fig. 7

From: Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps

Fig. 7

Role of TEPs in regulation of the Toll pathway. ac Male flies were pricked in the thorax with a needle dipped in a concentrated suspension of B. bassiana spores. Data were analyzed by log-rank test. Each panel shows a representative experiment of a minimum of three independent repeats. x-axis: time post-infection in days; y-axis: percentage of living flies. a TEPq Δ , GNBP3 hades double mutant flies are highly susceptible to B. bassiana as compared to the w 1118 control line (P < 0.001) with a survival curve comparable to that of spz rm7 flies. TEPq Δ or GNBP3 hades single mutant flies show a milder phenotype (P = 0.004 and P < 0.001, respectively, as compared to the double mutants). b TEPq Δ , ModSP 1 double mutant flies are highly susceptible to B. bassiana as compared to the w 1118 control line (P < 0.001) with a survival curve comparable to that of spz rm7 flies. TEPq Δ or ModSP 1 simple mutant flies show a milder phenotype (P < 0.001 for both as compared to the double mutant). c psh 1 , TEPq Δ double mutant flies show an increased susceptibility to B. bassiana compared to the w 1118 wild-type flies (P < 0.001). The survival curve is comparable to that of spz rm7 flies. TEPq Δ or psh 1 single mutant flies show a milder phenotype (P = 0.002 and P = 0.005, respectively), as compared to the double mutants. d Female flies were pricked in the thorax with a needle dipped in a concentrated suspension of B. bassiana spores, and the expression of Drs was measured 24 h post-infection. Values represent the mean ± SE of three independent experiments and were analysed using the Mann-Whitney test (two-sided). Drs expression for all the genotypes tested except for GNBP3 Δ was significantly lower than in the wild-type w 1118 flies, as indicated by asterisks in the chart (0.01 < P < 0.03 for all cases). There were no statistically significant differences between TEPq Δ flies and other compound knockouts (i.e. TEPq Δ , GNBP3 hades or TEPq Δ , ModSP 1 or psh 1 , TEPq Δ.) e Drs expression in response to injection of purified proteases of B. subtilis. No statistically significant differences were observed between the TEPq Δ and the wild type w 1118. f Drs expression in unchallenged male flies of the indicated genotypes. Values represent the mean ± SE of three independent experiments and were analysed using the Mann-Whitney test (two-sided). Overexpression of ModSP induces Drs expression to the same levels in the presence or absence of TEPs

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