Fig. 7

DNA marker sp16 is located in a W-linked region. a Results of agarose gel electrophoresis of PCR products amplified by DNA marker sp16 (sp16). Actin from D. rotundata (Dr-Actin) served as a control to show that template DNA was present for all samples. NF non-flowering. b Bar graphs showing the correspondence of sp16 genotypes (sp16 PCR product Amplified or Not amplified) with the sex of F1 progeny derived from a cross between P3 and P4 and phenotyped over 2 years (2014 and 2015). Color codes indicate sex manifestation of the plants during the 2-year period, disregarding the yearly order (i.e., plants showing sex changes from male [2014] to female [2015] and female [2014] to male [2015] were combined and are indicated by ♀/♂). Monoecy is indicated by (♀/♂). NF non-flowering. c The same as b but for F1 progeny obtained from a separate cross involving TDr04-219 and P4