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Fig. 1. | BMC Biology

Fig. 1.

From: Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?

Fig. 1.

Hydrogel-based clearing methods and their application to whole-brain and intact-bone clearing. a The protein retention mechanism and the lipid removal process. Prior to lipid removal, the proteins are anchored to a hydrogel scaffold via PFA-mediated binding. During the clearing process, the detergent selectively removes light scattering lipids, while proteins remain locked in place by the hydrogel. Following lipid removal, the tissue is rendered transparent. b The clearing chamber and its components. With the use of continuous conductive flow, the clearing process is accelerated (~ 2–3× faster) and can clear a whole brain or an entire bone. From [17]. Reprinted with permission from AAAS. c Maximum intensity projection of a PACT-cleared whole mouse brain (Thy1-YFP) and a digital section (1 mm deep). d Maximum intensity projection of a mouse femur cleared using Bone CLARITY, and a digital section (0.4 mm deep). The green channel represents auto-fluorescence, while the red channel represents cells that express Sox9. The images in c and d were captured using a commercial and custom light-sheet microscope, respectively. From [17]. Reprinted with permission from AAAS

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