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Fig. 2 | BMC Biology

Fig. 2

From: SKIP controls flowering time via the alternative splicing of SEF pre-mRNA in Arabidopsis

Fig. 2

SKIP is required for the transcriptional activation of FLC and its homologs to repress flowering. Ten-day-old seedlings were used to examine flowering time-related gene expression by quantitative reverse transcription (qRT)-PCR. a, c FLC expression in WT plants, skip-1, and three of the skip-1 complemented transgenic lines described in Fig. 1 under LD (a) and SD (c) conditions. b, d MAF expression in WT and skip-1 plants under LD (b) and SD (d) conditions. e, f, h, i SOC1 and FT expression in WT plants, skip-1, and the three complemented transgenic lines under LD (e and f) and SD (h, i) conditions. g, j TSF and FD expression in WT and skip-1 plants under LD (g) and SD (j) conditions. ACTIN 2 (ACT2) was used as an endogenous control. The values are the mean ± s.d. One-way analysis of variance (ANOVA; Tukey’s multiple comparison test) was performed for data in a, c, e, f, h, and i, and two-tailed unpaired t test was performed for data in b, d, g, and j. Statistically significant differences are indicated by different lowercase letters (P < 0.05). There are statistically significant differences between all non-identical letters

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