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Fig. 5 | BMC Biology

Fig. 5

From: SKIP controls flowering time via the alternative splicing of SEF pre-mRNA in Arabidopsis

Fig. 5

Splicing of SEF pre-mRNA regulates FLC expression. a Schematic representation of the constructs carrying the full-length CDS of SEF (SEFc), WT CDS with the first intron of SEF (wtSEF IR ), and mutated SEF sequence with the first intron (mSEF IR ) driven by the 35S promoter. In the mSEF IR construct, the 5'ss and 3'ss of the first intron were mutated, as shown in red. UTR represents untranslated region. I1, I2, and I3 represent introns 1, 2, and 3. E1, E2, E3, and E4 represent exons 1, 2, 3, and 4. The black arrows indicate the location of primers for RT-PCR. b SEF and FLC expression in WT plants, sef-2, and sef-2 transgenic lines carrying 35S:SEFc, 35S:wtSEF IR , or 35S:mSEF IR under LD and SD conditions as determined by RT-PCR. c, d SEF expression in WT plants, sef-2, and sef-2 transgenic lines carrying 35S:SEFc, 35S:wtSEF IR , or 35S:mSEF IR under LD and SD conditions as determined by qRT-PCR. e FLC expression in WT plants, sef-2, and sef-2 transgenic lines carrying 35S:SEFc, 35S:wtSEF IR , or 35S:mSEF IR under LD and SD conditions as determined by qRT-PCR. ACT2 was used as an endogenous control. The data are the mean ± s.d. (n = 3) in ce. SEF-Aa and SEF-Ab represent the mature mRNA and transcript containing the first and second introns of the SEF-A fragment (as shown in Fig. 5a). C5-15, C13-5, and C22-8 are sef-2 transgenic lines carrying the 35S:SEFc construct; W2-2, W23-7, and W33-2 are sef-2 transgenic lines carrying the 35S:wtSEF IR construct; and M2-13, M33-13, and M39-8 are sef-2 transgenic lines carrying the 35S:mSEF IR construct

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