Fig. 6
From: Role of PCNA and RFC in promoting Mus81-complex activity
Sensitivity of yeast strains to DNA-damaging agents. a Spot test assay. Serial dilutions (101, 102, 103, 104, and 105 cells) of isogenic wild type and single or double mutant yeast strains were spotted on YPD plates containing the indicated concentrations of CPT, HU, MMS, CisPT, or ZEO. b Colony formation assay. The diluted cells (biological triplicates for each strain) were spread onto YPAD plates containing selected drugs (CPT (1 μg/mL), MMS (0.0025%), HU (30 mM)) and the colonies were counted after 2–4 days of incubation at 30 °C. P values were calculated using two-tailed unpaired homoscedastic t-test. *Raw data provided in Additional file 6