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Fig. 3 | BMC Biology

Fig. 3

From: Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins

Fig. 3

The effect of light and loss of light on spontaneous movement of GtACR1 embryos. a, c, e The amount of movement displayed by individual embryos, in the 5 s before light onset (‘5 s before’), in the first 5 s after light onset (‘First 5 s’) and in the first 5 s after light offset (‘5 s after’). Different intensities of blue (a), green (c) and red (e) light were tested. Controls refer to siblings lacking eYFP expression. The line colour of each plot corresponds to the light colour employed for illumination. b, d, f The mean amount of movement relative to the period before light onset. A negative value indicates inhibition of spontaneous movement, whereas a positive value indicates elevated levels of movement. Mean differences and 95% CIs are reported alongside P values from Wilcoxon tests. Blue (b) and green (d) light both affect spontaneous movement. Red light has a small effect (f)

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