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Fig. 4 | BMC Biology

Fig. 4

From: Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins

Fig. 4

The effect of light and loss of light on spontaneous movement of GtACR2 embryos. a, c, e The amount of movement displayed by individual embryos, in the 5 s before light onset (‘5 s before’), in the first 5 s after light onset (‘First 5 s’) and in the first 5 s after light offset (‘5 s after’). Three different intensities of blue (a) and green (c) light and one intensity of red light (e) were tested. The colour of the lines indicates the colour of light used for illumination. Controls for each experiment were siblings not expressing eYFP. b, d, f The mean amount of movement relative to the period before light onset. A negative value indicates inhibition of spontaneous movement, whereas a positive value indicates elevated levels of movement. Mean differences and 95% CIs are reported; all P values are results of Wilcoxon tests. Blue (b) light affected spontaneous movement at medium and high intensities, whereas green light only had a weak effect at high intensity (d). Red light had no effect

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