Table 1 Oligonucleotide primers used to amplify and sequence the rbcL, matK, and ITS markers and their associated PCR thermocycling regimes
Marker | Primer | Source | PCR conditions |
|---|---|---|---|
rbcL | aF | Pryer et al., 2001 [55] | 5 min of initial denaturation at 80 °C; 30 cycles: 95 °C 1 min, 60 °C 1 min, 65 °C 2 min; final extension 5 min |
1379R | Pryer et al., 2001 [55] | ||
matK | matK-xf | Ford et al., 2009 [56] | 5 min of initial denaturation at 95 °C; 35 cycles: 95 °C 30 s, 49 °C 1 min, 72 °C 1 min 40 s; final extension 7 min |
matK-MALP | Dunning and Savolainen, 2010 [57] | ||
ITS | ITS5a | Stanford et al., 2000 [58] | 6 min of initial denaturation at 95 °C; 35 cycles: 95 °C 30 s, 49 °C 1 min, 72 °C 1 min 40 s; final extension 7 min |
ITS4 | White et al., 1990 [59] |