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Table 1 Oligonucleotide primers used to amplify and sequence the rbcL, matK, and ITS markers and their associated PCR thermocycling regimes

From: Spatial phylogenetics of the native California flora

Marker Primer Source PCR conditions
rbcL aF Pryer et al., 2001 [55] 5 min of initial denaturation at 80 °C; 30 cycles: 95 °C 1 min, 60 °C 1 min, 65 °C 2 min; final extension 5 min
1379R Pryer et al., 2001 [55]
matK matK-xf Ford et al., 2009 [56] 5 min of initial denaturation at 95 °C; 35 cycles: 95 °C 30 s, 49 °C 1 min, 72 °C 1 min 40 s; final extension 7 min
matK-MALP Dunning and Savolainen, 2010 [57]
ITS ITS5a Stanford et al., 2000 [58] 6 min of initial denaturation at 95 °C; 35 cycles: 95 °C 30 s, 49 °C 1 min, 72 °C 1 min 40 s; final extension 7 min
ITS4 White et al., 1990 [59]
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