Spatial phylogenetics of the native California flora

Table 1 Oligonucleotide primers used to amplify and sequence the rbcL, matK, and ITS markers and their associated PCR thermocycling regimes

Marker	Primer	Source	PCR conditions
rbcL	aF	Pryer et al., 2001 [55]	5 min of initial denaturation at 80 °C; 30 cycles: 95 °C 1 min, 60 °C 1 min, 65 °C 2 min; final extension 5 min
rbcL	1379R	Pryer et al., 2001 [55]
matK	matK-xf	Ford et al., 2009 [56]	5 min of initial denaturation at 95 °C; 35 cycles: 95 °C 30 s, 49 °C 1 min, 72 °C 1 min 40 s; final extension 7 min
matK	matK-MALP	Dunning and Savolainen, 2010 [57]
ITS	ITS5a	Stanford et al., 2000 [58]	6 min of initial denaturation at 95 °C; 35 cycles: 95 °C 30 s, 49 °C 1 min, 72 °C 1 min 40 s; final extension 7 min
ITS	ITS4	White et al., 1990 [59]

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