Fig. 2

Molecular modeling of the disordered segment and sequence alignment. a Molecular modeling calculations showing the flexibility of the disordered segment of FlgE from C. jejuni. Representative structures, plotted every 10th structure, are sorted and colored by energy for the loop model population (scale ranges from blue (500 Rosetta energy units; REU) to red (850 REU)). b Cartoon representation of the 3D structure of FlgE from C. jejuni, in green, with the “L-shaped” (L-Stretch) disordered segment connecting D0 to D1 obtained, and FlgE and FlgG from S. enterica, in cyan and in purple, respectively. c and d Sequence alignments of the rod/hook disordered segment from FlgE and FlgG of gram-negative and -positive bacteria with the position of the disordered segment marked by an arrow. In FlgE (c), the segments have different lengths, while in FlgG (d), the length is conserved. The red stars show the position of the conserved residues. For comparison, the sequence of FlgE from C. jejuni is marked in blue. Camje: C. jejuni, Salen: S. enterica, Escco: Escherichia coli, Pseae: Pseudomonas aeruginosa, Vibch: Vibrio cholerae, Caucr: Caulobacter crescentus, Borbu: Borrelia burgdorferi, Aquae: Aquifex aeolicus, Bacsu: Bacillus subtilis, Gilap: Gilliamella apicola, Wiggl: Wigglesworthia glossinidia, Sodgm; Sodalis glossinidius. Sequence alignment was done with Clustal Omega [30]