Fig. 4From: An intrinsically disordered linker controlling the formation and the stability of the bacterial flagellar hookMotility and flagella of Salmonella expressing FlgE ID-Rod-Stretch mutant proteins. a Motility for strains inoculated into soft tryptone agar that expressed FlgE proteins with conserved amino acid residues exchanged with alanine residues, as indicated. The plate was incubated at 30 °C for 6 h. Western blot analysis of FlgE proteins exported into the supernatant broth (b) or remaining in the cell pellet fractions after centrifugation (c) for strains grown in LB at 37 °C. Relative band densities (%) compared to wild-type are indicated. Polyclonal antibodies were used. Wild-type FlgE is 42 kDa. Electron micrographs of cells expressing wild-type FlgE (d), FlgE K32A (e), or FlgE T28A, G30A, F31A, K32A, F38A, and M41A mutant proteins (f). Scale bars, 1 μmBack to article page