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Fig. 6 | BMC Biology

Fig. 6

From: An intrinsically disordered linker controlling the formation and the stability of the bacterial flagellar hook

Fig. 6

Motility and biosynthesis of flagella is decreased for Salmonella ID-Rod-Stretch mutant strains. a Motility phenotype in soft tryptone agar of a ΔflgE null mutant strain that harbored plasmids carrying flgE mutant genes. FlgE proteins were encoded with five amino acid residues exchanged with five alanine residues. The plate was incubated at 30 °C for 6 h. Box plot (b) displaying the distribution of the numbers of flagella per cell, for > 30 cells of each strain examined using transmission electron microscopy. Western blotting analysis of the amount of FlgE and FliC in the cell (c) and exported (d) into the culture supernatant broth for cultures of the strains grown in LB to early stationary phase at 37 °C. Polyclonal antibodies were used. The Fla- strain does not produce flagella. Relative band densities (%) compared to wild-type are indicated. FliC is 51 kDa and wild-type FlgE is 42 kDa

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